| Related Categories | Antibody Characterization and Analysis, SILuMAb and SigmaMAb Antibody Standards for Mass Spec Analysis, 分子生物学, 生化试剂, 用于质谱分析的多肽及蛋白标准品, 蛋白质及衍生物, 蛋白质组学, 质谱 更多... |
| recombinant | expressed in CHO cells |
| antibody product type | primary antibodies |
| assay | ≥90% (SDS-PAGE) |
| packaging | vial of 100 μg |
| shipped in | wet ice |
| storage temp. | −20°C |
Adalimumab Heavy Chain: EVQLVESGGGLVQPGRSLRLSCAAS
FTISRDNAKNSLYLQMNSLRAEDTA
GALTSGVHTFPAVLQSSGLYSLSSV
TCVVVDVSHEDPEVKFNWYVDGVEV
ELTKNQVSLTCLVKGFYPSDIAVEW
Adalimumab Light Chain:
DIQMTQSPSSLSASVGDRVTITCRA
Preparation Note
Produced utilizing enriched media containing stable isotope labeled amino acids are 13C6, 15N4-labeled Arginine and 13C6, 15N2-labeled Lysine.
SILu™Mab Adalimumab is designed to be used as a internal standard for analysis of Adalimumab in human serum.
Reconstitution
Each vial of Adalimumab contains the labeled antibody in a lyophilized form containing phosphate buffered saline.
SILu™Mab Adalimumab recovery is maximized when 0.1% formic acid is used for reconstitution of the lyophilized product.Reconstitution with other solvents may reduce recovery. Do not freeze after reconstitution.
• Briefly centrifuge the vial at ~10,000 × g to collect the product at the bottom of the vial.
• Add 500 μL of ultrapure water containing 0.1% formic acid to the vial.
• Mix the contents by gently inverting the vial a minimum of 5 times.
• Allow the vial to stａnd at room temperature for at least 15 minutes and repeat mixing by inversion.
Poster: Multiplex Quantification of Infliximab and Adalimumab in Human Serum by LC-MS/MS Using Full-Length Stable Isotope Labeled Internal Standards
General description
Adalimumab Stable-Isotope Labeled Monoclonal Antibody is a recombinant, stable isotope labeled, monoclonal antibody which incorporates [13C6, 15N4]-Arginine and [13C6, 15N2]-Lysine. Expressed in CHO cells, SILuMAb Adalimumab is designed to be used as an internal standard for analysis of Adalimumab in human serum. Each vial of SILuMAb Adalimumab contains the labeled antibody lyophilized from a solution of phosphate buffered saline. Vial content was determined by measuring A280 and using an extinction coefficient (E0.1%) of 1.4.
Adalimumab is for research use only and is not intended for diagnostic or therapeutic use.
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